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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in lots of tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was located to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital function in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two additional splice variants ended up documented but in depth nucleotide sequence details is just not but readily available.
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Blocking peptides are peptides that bind especially towards the focus on antibody and block antibody binding. These peptide generally contains the epitope identified from the antibody.
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This guide outlines even more probable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
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This manual outlines further more attainable optimization of cellular and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide not bind for the epitope about the focus on protein. By evaluating the staining in the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose associates are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which lead on the temporal coordination of each mitotic celebration. Cyclin E varieties a fancy with and features being a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates in the G1-S stage boundary and is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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